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Operon Calculator
Design Job Title / Operon Name


Select an Organism

Upload your Annotated Operon Sequence in Genbank Format:
OR Enter your operon's sequence information below

Select a Promoter (enter a promoter sequence, or select one from the list)
Promoter Sequence:

Download Genbank

Protein Coding Sequence #1
CDS Name:
RBS Sequence[?]
Ribosome Binding Site Sequence: enter the nucleotide sequence that controls a mRNA's translation initiation rate, using A/G/C/T/U. A complete RBS sequence is 35 nucleotides long.
:

CDS Sequence (nt only) [?]
Protein Coding Sequence: enter a nucleotide sequence (at least 50 bp) that encodes a protein, using A/G/C/T/U. Begins with a start codon (ATG/GTG/TTG/CTG). (required)
:

Protein Coding Sequence #2
CDS Name:
RBS Sequence[?]
Ribosome Binding Site Sequence: enter the nucleotide sequence that controls a mRNA's translation initiation rate, using A/G/C/T/U. A complete RBS sequence is 35 nucleotides long.
:

CDS Sequence (nt only) [?]
Protein Coding Sequence: enter a nucleotide sequence (at least 50 bp) that encodes a protein, using A/G/C/T/U. Begins with a start codon (ATG/GTG/TTG/CTG). (required)
:

Protein Coding Sequence #3
CDS Name:
RBS Sequence[?]
Ribosome Binding Site Sequence: enter the nucleotide sequence that controls a mRNA's translation initiation rate, using A/G/C/T/U. A complete RBS sequence is 35 nucleotides long.
:

CDS Sequence (nt only) [?]
Protein Coding Sequence: enter a nucleotide sequence (at least 50 bp) that encodes a protein, using A/G/C/T/U. Begins with a start codon (ATG/GTG/TTG/CTG). (required)
:
Add Protein CDS #4 Remove CDS #3

Select a Transcriptional Terminator (enter a sequence, or select one from the list)
Terminator Sequence:

Download Genbank
Design Jobs: 2 queued, 3 currently running
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We gratefully acknowledge research funding from the Air Force Office of Scientific Research, the National Science Foundation, the Office of Naval Research, and an Amazon AWS Research Grant.
Computational resources are provided by the AWS Elastic Compute Cloud.