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Operon Calculator
Operon Examples
Design Job Title / Operon Name

Active Design Rules
Improve mRNA Stability Remove Undesired Translation Products
Remove Internal Promoters Remove Ribosomal Pause Sites
Remove Internal Terminators Remove Genetic Instability Sites
Remove Repetitive DNA

Synonymous Codon Usage Table
View Codon Table

Select a Promoter (enter a promoter sequence, or select one from the list)
Promoter Sequence:

Download Genbank

Protein Coding Sequence #1
CDS Name:
Target Translation Rate [?]
Target Translation Initiation Rate: select the desired rate of translation initiation on a proportional scale from 0.1 to 100,000 or more. (required)
:
Proportional scale (1 to 100,000+)
CDS Sequence (aa or nt) [?]
Protein Coding Sequence: enter a nucleotide or amino acid sequence (at least 50 bp or 17 aa) that encodes a polypeptide, using A/G/C/T/U or the 1-letter amino acid abbreviation. (required)
:

Protein Coding Sequence #2
CDS Name:
Target Translation Rate [?]
Target Translation Initiation Rate: select the desired rate of translation initiation on a proportional scale from 0.1 to 100,000 or more. (required)
:
Proportional scale (1 to 100,000+)
CDS Sequence (aa or nt) [?]
Protein Coding Sequence: enter a nucleotide or amino acid sequence (at least 50 bp or 17 aa) that encodes a polypeptide, using A/G/C/T/U or the 1-letter amino acid abbreviation. (required)
:

Protein Coding Sequence #3
CDS Name:
Target Translation Rate [?]
Target Translation Initiation Rate: select the desired rate of translation initiation on a proportional scale from 0.1 to 100,000 or more. (required)
:
Proportional scale (1 to 100,000+)
CDS Sequence (aa or nt) [?]
Protein Coding Sequence: enter a nucleotide or amino acid sequence (at least 50 bp or 17 aa) that encodes a polypeptide, using A/G/C/T/U or the 1-letter amino acid abbreviation. (required)
:
Add Protein CDS #4 Remove CDS #3

Select a Transcriptional Terminator (enter a sequence, or select one from the list)
Terminator Sequence:

Download Genbank

Select an Organism

Design Jobs: 2 queued, 4 currently running
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We gratefully acknowledge research funding from the Air Force Office of Scientific Research, the National Science Foundation, the Office of Naval Research, and an Amazon AWS Research Grant.
Computational resources are provided by the AWS Elastic Compute Cloud.